Rat Anti-Mouse CCL8 Monoclonal Antibody (CBP3713)

Description

CCL8, also known as MCP-2, is a beta chemokine originally isolated from human MG-63 osteosarcoma cells stimulated by IL-1β. CCL8 (MCP-2) has a structure and function similar to MCP-1 and MCP-3. It is usually co-induced with those chemokines in monocytes. IFNγ is a strong inducer of CCL8 (MCP-2) in monocytes and fibroblasts. CCL8 (MCP-2) can be processed and secreted into truncated chemokine CCL8 (6-75) in monocytes and fibroblasts. This isoform will not chemically attract monocytes, but will bind to the CCR2 receptor and induce its internalization. As a result, CCL8 (MCP-2) inhibited MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling, and antagonized several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP- 3 / CCL7). CCL8 (MCP-2) is highly expressed in the skin, and it is an agonist of the chemokine receptor CCR8, not an agonist of CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a key regulator of Th2 homing, which drives IL-5 mediated chronic allergic inflammation. In addition, CCL8 (MCP-2) binds to the CCR5 receptor with high affinity and inhibits the replication of type 1 human immunodeficiency virus. CCL8 (MCP-2) is highly expressed in different human diseases and is a potential biomarker. Diagnosis of graft versus host disease and tuberculosis.

Immunogen

Recombinant protein

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2b, κ

Clone Number

CBP3713

Applications

ELISA

Application Notes

ELISA (0.5-2.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CCL8

Official Name

CCL8

Full Name

Chemokine (C-C motif) ligand 8

Alternative Names

SCYA10; MCP2; Small-inducible cytokine A8; 6-76; Monocyte chemotactic protein 2; HC14; SCYA8; C-C motif chemokine 8; MCP-2; Monocyte chemoattractant protein 2

Gene ID

6355(Human)

Uniprot ID

P80075(Human)

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5. Rom S, et al. 2010. FASEB J. 24:2292.
6. Struyf S, et al. 2009. Eur. J. Immunol. 39:843.
7. Islam SA, et al. 2011. Nat. Immunol. 12:167.