Mouse Anti-Nitrotyrosine Monoclonal Antibody (CBP4230)

Description

Nitrotyrosine is the product of tyrosine nitration, which is mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a sign of inflammation and nitric oxide (NO) production. It has been detected under a large number of pathological conditions and is considered to be a sign of NO-dependent, nitrifying stress induced by reactive nitrogen species. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues. Nitrification prevents phosphorylation of tyrosine residues, which is important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaque, chronic renal failure and keratoconus. Since nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. The nitrotyrosine antibody detects proteins and peptides containing nitrotyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins, determining protein nitrification and measuring the level of nitrated proteins in tissues and samples.

Immunogen

KLH modified with nitrotyrosine.

Species Reactivity

Human; Mouse; Rat; Dog

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP4230

Applications

WB; ELISA

Application Notes

WB: 0.5 µg/mL, ELISA: 0.05 µg/mL

Conjugation

Unconjugated

Form

Colorless liquid

Formulation

Lyophilized from 1 ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Preservatives

Yes

Shipping

Gel Pack

Storage

-20°C

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

Nitrotyrosine