Nitric Oxide Detection Kit, Species Independent

[CAT#: NK1120FY009]

Colorimetric detection of nitrate and nitrite

Applications:: Indirect Quantitative Assay

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Request Pricing

Inquiry

Product Overview

Description

The purpose of the Nitric Oxide Detection Kit is to quantify the amounts of nitrate and nitrite found in various samples. The total of nitrate (-NO3) and nitrite (-NO2) yields the nitric oxide content. To create standard curves for the test, nitrate and nitrite standards are supplied; all samples must be read off the corresponding standard curve.

Species Reactivity

Wide Species

Applications

Indirect Quantitative Assay

Relevant Diseases

Neurological Disorders

Properites

Sensitivity

In most cases, the sensitivity is below 2.6 µM in the Nitrite and 1.02 µM in the Total Nitric Oxide.

Platform

Microplate

Detection Method

Colorimetric Assay

Sample Type

Buffer, Cell lysates, Plasma, Saliva, Serum, Tissue Culture Media, Urine, Water

Assay Type

Detection Kits

Assay Time

30 minutes

Linear Range

3.12 - 200 µM

Components

Clear 96 Well Plates 1 Each
Nitrate Standard 200 µl
Nitrite Standard 200 µl
Assay Buffer 60 ml
NADH Concentrate 1.2 ml
Nitrate Reductase 1 Vial
Enzyme Stabilization Buffer 1 ml
Color Reagent A 5 ml
Color Reagent B 5 ml

Preparation and Storage