Nitric Oxide Detection Kit, Species Independent
[CAT#: NK1120FY009]
Colorimetric detection of nitrate and nitrite
- Applications:
- Indirect Quantitative Assay
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The purpose of the Nitric Oxide Detection Kit is to quantify the amounts of nitrate and nitrite found in various samples. The total of nitrate (-NO3) and nitrite (-NO2) yields the nitric oxide content. To create standard curves for the test, nitrate and nitrite standards are supplied; all samples must be read off the corresponding standard curve.
Species Reactivity
Wide Species
Applications
Indirect Quantitative Assay
Relevant Diseases
Neurological Disorders
Properites
Sensitivity
In most cases, the sensitivity is below 2.6 µM in the Nitrite and 1.02 µM in the Total Nitric Oxide.
Platform
Microplate
Detection Method
Colorimetric Assay
Sample Type
Buffer, Cell lysates, Plasma, Saliva, Serum, Tissue Culture Media, Urine, Water
Assay Type
Detection Kits
Assay Time
30 minutes
Linear Range
3.12 - 200 µM
Components
Clear 96 Well Plates 1 Each
Nitrate Standard 200 µl
Nitrite Standard 200 µl
Assay Buffer 60 ml
NADH Concentrate 1.2 ml
Nitrate Reductase 1 Vial
Enzyme Stabilization Buffer 1 ml
Color Reagent A 5 ml
Color Reagent B 5 ml
Nitrate Standard 200 µl
Nitrite Standard 200 µl
Assay Buffer 60 ml
NADH Concentrate 1.2 ml
Nitrate Reductase 1 Vial
Enzyme Stabilization Buffer 1 ml
Color Reagent A 5 ml
Color Reagent B 5 ml
Preparation and Storage
Shipping
Blue Ice
Storage
4°C
Research Use Only
For research use only
Target Details
Target
NO
Official Name
Nitric Oxide
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
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