NQO1 (human intracellular) ELISA Kit, Mammalian

Description

NAD(P)H: Quinone receptor oxidoreductase 1 (NQO1) is a multifunctional antioxidant and a multi-purpose cytoprotective agent that can protect cells from carcinogenic oxidative damage and stabilize the tumor suppressor p53 protein. Under high glycolysis levels, NQO1 can stabilize and protect p53 from ubiquitin-dependent degradation (NADH-dependent), and it can also increase NAD + / NADH levels. NAD + and NADH play a key role in cell energy metabolism, and the imbalance of NAD + / NADH ratio is related to metabolic syndrome. In humans, NQO1 is expressed at high levels in adipocytes, and its expression level is positively correlated with obesity, glucose tolerance and liver function abnormalities. Therefore, NQO1 can provide a basis for the treatment of metabolic syndrome. The assay is a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human NQO1 in cells. The NQO1 specific monoclonal antibody has been pre-coated on 96-well microtiter plates. The standards and samples are transferred into the wells to bind the coated antibody. After thorough washing to remove unbound compounds, NQO1 is recognized by adding a purified polyclonal antibody (detection antibody) specific to NQO1. After removing the excess polyclonal antibody, add HRP-conjugated anti-rabbit IgG (detector). After the final washing, the substrate 3,3',5,5'-tetramethylbenzidine (TMB) was used to quantify the peroxidase activity. After acidification, the intensity of the color reaction was measured at 450 nm, and the intensity was proportional to the concentration of NQO1 in the sample. This ELISA is dedicated to the measurement of natural and recombinant human NQO1. It does not interact with human adiponectin, human RBP4, human Nampt, human vaspin, human pregranulin, human resistin, human clusterin, human ANGPTL3, human CTRP5, human IL-33, human leptin, human GPX3, human NMNAT2, human sirtuin cross-reaction. 1. Human FTO, mouse Nampt, rat Nampt. The measurement range is 0.313 - 20 ng NQO1/ml, and the detection limit is 100 pg/ml (based on adding two standard deviations to the average value of (50) zero standards).
This ELISA kit is used for the in vitro quantitative determination of human NQO1 in the range of 0.313 - 20 ng NQO1/ml and a detection limit of 100 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Detection Range

0.313 - 20 ng NQO1/ml

Components

• Pre-coated Microtiter Plate
• Wash Buffer (10X)
• Diluent (5X)
• Lysis Buffer (10X)
• Detection Antibody
• Detector 100X (Hrp conjugated anti-IgG)
• Human NQO1 Standard (lyophilized, 40 ng)
• Human NQO1 QC Sample (lyophilized)
• TMB Substrate Solution
• Stop Solution
• Plate Sealers

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only

Target

NQO1

Official Name

NQO1

Full Name

NAD(P)H dehydrogenase [quinone] 1

Alternative Names

NQO1; DHQU; DIA4; DTD; NMOR1; NMORI; QR1; NAD(P)H dehydrogenase; NAD(P)H quinone dehydrogenase 1

Gene ID

1728(Human); 18104(Mouse); 24314(Rat)

Uniprot ID

P15559(Human); Q64669(Mouse); P05982(Rat)