Lenti of Rat NAD(P)H dehydrogenase, quinone 1 (Nqo1) (NM_017000) ORF clone, mGFP Tagged

Description

This is a rat NQO1 mGFP tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

NQO1

Species

Rat

Insert Size (bp)

822 bp

Tag

mGFP

Type

Expression Vector/ Viral Particle

Vector

pLenti-C-mGFP-P2A-Puro

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGCGGTGAGAAGAGCCCTGATTGTATTGGCCCACGCAGAGAGGACATCATTCAACTATGCCATGAAGGAGGCTGCTGTGGAGGCTCTGAAGAAGAAAGGATGGGAGGTGGTCGAATCTGACCTCTATGCTATGAACTTTAACCCCCTCATTTCCAGAAACGACATCACAGGGGAGCCGAAGGACTCGGAGAACTTTCAGTACCCTGTTGAGTCATCTCTGGCGTATAAGGAAGGCCGCCTGAGCCCGGATATTGTAGCTGAACAGAAAAAGCTGGAAGCTGCAGACCTGGTGATATTTCAGTTCCCATTGTATTGGTTTGGGGTGCCCGCCATTCTGAAAGGCTGGTTTGAGAGAGTGCTTGTAGCAGGATTCGCCTACACGTATGCCACCATGTATGACAAGGGTCCTTTCCAGAATAAGAAGACCTTGCTTTCCATCACCACCGGGGGCAGCGGCTCCATGTACTCTCTGCAGGGTGTCCACGGGGACATGAACGTCATTCTCTGGCCAATTCAGAGTGGCATTCTGCGCTTCTGTGGCTTCCAGGTCTTAGAACCTCAACTGGTGTACAGCATTGGCCACACCCCACCCGATGCCCGCGTGCAGGTCCTGGAAGGGTGGAAGAAGCGTCTGGAGACTGTCTGGGAGGAGTCACCACTCTACTTTGCTCCAAGCAGCTTGTTTGACCTAAACTTCCAGGCAGGATTCTTACTGAAAAAAGAGGTTCAAGAGGAGCAGAAAAAGAACAAGTTTGGCCTTTCTGTGGGCCATCATTTGGGCAAGTCCATTCCAGCCGACAACCAGATCAAAGCTAGAAAAACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neurotransmitter

Relevant Diseases

Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

NQO1

Official Name

NQO1

Full Name

NAD(P)H dehydrogenase [quinone] 1

Alternative Names

NQO1; DHQU; DIA4; DTD; NMOR1; NMORI; QR1; NAD(P)H dehydrogenase; NAD(P)H quinone dehydrogenase 1

Gene ID

1728(Human); 18104(Mouse); 24314(Rat)

Uniprot ID

P15559(Human); Q64669(Mouse); P05982(Rat)