Rat heparan sulfate proteoglycan 2 (HSPG2) ELISA kit

[CAT#: NRZP-0722-ZP231]

For the quantitative determination of rat heparan sulfate proteoglycan 2 (HSPG2) concentrations in serum, plasma, cell culture supernates, tissue homogenates, bronchoalveolar lavage fluid, urine.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of rat HSPG2.

Species Reactivity

Rat

Marker

Heparan sulfate proteoglycan 2

Properites

Sensitivity

In most cases, the sensitivity is below 3.9 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

15.6 pg/ml-1000 pg/ml

Max Emission

450 nm

Target Details

Target

Heparan Sulfate Proteoglycan 2 / Perlecan

Official Name

Hspg2

Full Name

heparan sulfate proteoglycan 2

Alternative Names

RP11-132G19.2, PLC, PRCAN, SJA, SJS, SJS1, Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia)|endorepellin (domain V region)|heparan sulfate proteoglycan of basement membrane|perlecan proteogly

Gene ID

313641(Rat)

Uniprot ID

O08591(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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