Human heparan sulfate proteoglycan 2 (HSPG2) ELISA kit

[CAT#: NRZP-0722-ZP226]

For the quantitative determination of human heparan sulfate proteoglycan 2 (HSPG2) concentrations in serum, plasma.

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human HSPG2.

Species Reactivity

Human

Marker

Heparan sulfate proteoglycan 2

Properites

Sensitivity

In most cases, the sensitivity is below 15.6 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

62.5 pg/ml-4000 pg/ml

Max Emission

450 nm

Target Details

Target

Heparan Sulfate Proteoglycan 2 / Perlecan

Official Name

HSPG2

Full Name

heparan sulfate proteoglycan 2

Alternative Names

RP11-132G19.2, PLC, PRCAN, SJA, SJS, SJS1, Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia)|endorepellin (domain V region)|heparan sulfate proteoglycan of basement membrane|perlecan proteogly

Gene ID

3339(Human)

Uniprot ID

P98160(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

Publications