Rat Platelet glycoprotein 4(CD36) ELISA kit

[CAT#: NRZP-0722-ZP277]

For the quantitative determination of rat platelet glycoprotein 4 (CD36) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of rat CD36.

Species Reactivity

Rat

Marker

CD36 molecule (thrombospondin receptor)

Properites

Sensitivity

In most cases, the sensitivity is below 0.078 ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.312 ng/ml-20 ng/ml

Max Emission

450 nm

Target Details

Target

CD36

Official Name

CD36

Full Name

CD36 molecule

Alternative Names

FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10

Gene ID

29184(Rat)

Uniprot ID

Q07969(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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