Tissue Transglutaminase (tTG) ELISA Kit, Human

Description

Tissue transglutaminase (tTG) cross-links the protein between the ε-amino group of lysine residues and the γ-carboxamide group of glutamine residues, forming a molecule that is highly resistant to proteolysis (protein degradation) Intermolecular or intramolecular bonds. In addition to its cross-linking function, tTG also catalyzes other types of reactions, including deamidation, GTP binding/hydrolysis and isopeptidase activity. Tissue transglutaminase can be found in the intracellular and extracellular spaces of various types of tissues, and can be found in many different organs, including the heart, liver, and small intestine. The content of tTG in the cell is high in the cytoplasm, but a small amount can also be found in the nucleus and mitochondria. Our tTG ELISA kit is a sandwich ELISA assay for the quantitative determination of tTG in human serum, plasma and cell culture supernatant. The density of the color is directly proportional to the amount of tTG captured from the sample.
This ELISA kit is used for quantitative measurement of tTG in human serum, plasma, tissue homogenates and other biological fluids.

Species Reactivity

Human

Application Notes

This assay has high sensitivity and excellent specificity for detection of tTG. No significant cross-reactivity or interference between tTG and analogues was observed.

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Components

• Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only

Target

TGM2

Official Name

TGM2

Full Name

TGM2 Gene(Protein Coding) Transglutaminase 2

Alternative Names

Transglutaminase 2; C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase; Tissue Transglutaminase; Transglutaminase C; Transglutaminase H; EC 2.3.2.13; TGase C; TGase H

Gene ID

7052(Human); 21817(Mouse)

Uniprot ID

P21980(Human); P21981(Mouse)