Product
Ubiquitin Detection Kit, Species Independent
[CAT#: NK1120FY012]
Capture and detection of ubiquitinated proteins
- Applications:
- Binding Matrix/Quantitative WB (Western Blot)
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The Ubiquitin Detection kit facilitates the fast, effective capture and detection of ubiquitinated proteins from biological samples. The kit utilises a high capacity, high specificity ubiquitin binding matrix together with an easy-to-use purification system for less 'hands on time' and superior performance. Allows purification of mono- and poly-ubiquitinated proteins, independent of chain linkage or length, but not free ubiquitin. Highly adaptable, compatible with samples from a wide range of species and with a broad range of lysis buffers. Analysis by Western blotting or proteomic methods enables identification and assessment of ubiquitinated proteins of interest. Kit contains sufficient ubiquitin matrix to perform up to 20 assays.
Species Reactivity
Wide Species
Applications
Binding Matrix/Quantitative WB (Western Blot)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Properites
Platform
Spin Column
Sample Type
Cell lysates, Tissue
Assay Type
Detection Kits
Assay Time
1 Hour
Number Of Samples
20 samples
Components
StressXpress® ubiquitin matrix (50% slurry) 400 µl
StressXpress® columns 20 tubes
StressXpress® collection tubes 20 tubes
Ubiquitin monoclonal antibody (FK2) (HRP) 25 µl
StressXpress® columns 20 tubes
StressXpress® collection tubes 20 tubes
Ubiquitin monoclonal antibody (FK2) (HRP) 25 µl
Preparation and Storage
Shipping
Blue Ice
Storage
4°C
Research Use Only
For research use only
Target Details
References
1. Fulda, S., Rajalingam, K. & Dikic, I. EMBO molecular medicine 4, 545-56 (2012).
2. Shaid, S., Brandts, C. H., Serve, H. & Dikic, I. Cell death and differentiation 20, 21-30 (2013).
3. Husnjak, K. & Dikic, I. Annual review of biochemistry 81, 291-322 (2012).
4. Komander, D. & Rape, M. Annual Review of Biochemistry 81, 203-229 (2012).
5. Spasser, L. & Brik, A. Angewandte Chemie (International ed. in English) 51, 6840-62 (2012).
2. Shaid, S., Brandts, C. H., Serve, H. & Dikic, I. Cell death and differentiation 20, 21-30 (2013).
3. Husnjak, K. & Dikic, I. Annual review of biochemistry 81, 291-322 (2012).
4. Komander, D. & Rape, M. Annual Review of Biochemistry 81, 203-229 (2012).
5. Spasser, L. & Brik, A. Angewandte Chemie (International ed. in English) 51, 6840-62 (2012).
Publications
Publications (0)
Neural Antibodies
Kits
For Research Use Only. Not For Clinical Use.
