Product
Ubiquitin Interact Detection Kit, Species Independent
[CAT#: NK1120FY013]
Capture and detection of ubiquitin binding proteins
- Applications:
- Binding Matrix/Quantitative WB (Western Blot)
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The Ubiquitin Interact Detection kit facilitates the selective capture and detection of ubiquitin binding and associated proteins from cell lysates and tissue extracts. The kit utilises a high capacity, high specificity ubiquitin matrix together with an easy-to-use purification system for efficient isolation of ubiquitin binding proteins with minimal non-specific binding. Allows purification of mono- and poly-ubiquitin binding proteins, independent of chain linkage or length. Analysis by Western blotting or proteomic methods enables identification and assessment of captured proteins. Kit contains sufficient ubiquitin interact matrix to perform up to 20 binding assays.
Species Reactivity
Wide Species
Applications
Binding Matrix/Quantitative WB (Western Blot)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Properites
Platform
Spin Column
Detection Method
Colorimetric Assay
Sample Type
Cell lysates, Tissue
Assay Type
Detection Kits
Assay Time
1 Hour
Number Of Samples
20 samples
Components
StressXpress® ubiquitin interact matrix (50% slurry) 800 µl
StressXpress® columns 20 tubes
StressXpress® collection tubes 20 tubes
StressXpress® columns 20 tubes
StressXpress® collection tubes 20 tubes
Preparation and Storage
Shipping
Blue Ice
Storage
4°C
Research Use Only
For research use only
Target Details
References
1. Fulda, S., Rajalingam, K. & Dikic, I. EMBO molecular medicine 4, 545-56 (2012).
2. Shaid, S., Brandts, C. H., Serve, H. & Dikic, I. Cell death and differentiation 20, 21-30 (2013).
3. Husnjak, K. & Dikic, I. Annual review of biochemistry 81, 291-322 (2012).
4. Komander, D. & Rape, M. Annual Review of Biochemistry 81, 203-229 (2012).
5. Spasser, L. & Brik, A. Angewandte Chemie (International ed. in English) 51, 6840-62 (2012).
2. Shaid, S., Brandts, C. H., Serve, H. & Dikic, I. Cell death and differentiation 20, 21-30 (2013).
3. Husnjak, K. & Dikic, I. Annual review of biochemistry 81, 291-322 (2012).
4. Komander, D. & Rape, M. Annual Review of Biochemistry 81, 203-229 (2012).
5. Spasser, L. & Brik, A. Angewandte Chemie (International ed. in English) 51, 6840-62 (2012).
Publications
Publications (0)
Neural Antibodies
Kits
For Research Use Only. Not For Clinical Use.
