Product
Bioluminescence Cytotoxicity Assay Kit, Mammalian
[CAT#: NK1120FY198]
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The quantification of plasma membrane damage is classically used to assess cell death or cytotoxicity. Adenylate kinase (AK) is a ubiquitous protein found in all eukaryotic and prokaryotic cells. Once the plasma membrane is damaged, it will be quickly released into the culture medium. The bioluminescent cytotoxicity assay kit is based on the measurement of AK. This step is performed in a simple one-step method involving two chemical reactions. The first reaction is the conversion of ADP to ATP by adenylate kinase released by damaged cells. The second reaction uses luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or beta counter. The assay is very sensitive and can be performed fully automatically to achieve high throughput.
Species Reactivity
Mammalian
Relevant Diseases
Neurological Disorders
Properites
Detection Method
Luminescence
Components
• AK Detection Reagent (Lyophilized)
• AK Assay Buffer
• AK Assay Buffer
Preparation and Storage
Shipping
Gel Pack
Storage
-20°C
Research Use Only
For research use only
Target Details
Target
Bioluminescence
Official Name
Bioluminescence
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
