Choroid Plexus Organoid Modeling Service

The choroid plexus (ChP) is a unique and vital structure nestled deep within the brain. Often called the brain's "gatekeeper," it forms the crucial blood-cerebrospinal fluid barrier (BCSFB) and is the primary producer of cerebrospinal fluid (CSF) – the life-sustaining liquid that nourishes, protects, and cleanses our central nervous system. Understanding the ChP is key to unraveling brain development, function, health, and disease.

However, studying this intricate human structure directly presents significant challenges. Traditional models, like animal studies or simple 2D cell cultures, often fall short in capturing the complex, three-dimensional architecture and specific human physiology of the ChP and its barrier functions.

At Creative Biolabs, we bridge this gap. We've harnessed cutting-edge stem cell technology to generate sophisticated human Choroid Plexus Organoids, providing researchers like you with an unprecedented window into the workings of this critical neurointerface.

Why Choose Creative Biolabs' ChP Organoid Models?

Our ChP organoids are not just cell cultures; they are self-organizing 3D structures derived from human pluripotent stem cells (iPSCs) or embryonic stem cell line H9. They offer remarkable advantages:

• Human Relevance: Developed from human cells, our organoids provide insights directly applicable to human physiology and disease, reducing reliance on animal models.
• Complex Architecture: They recapitulate key structural features of the native ChP, including polarized epithelial layers forming cystic structures.
• Functional Barrier: Our organoids establish a functional BCSFB, crucial for studying transport, permeability, and barrier integrity.
• CSF-like Fluid Production: Organoids generate and secrete fluid into their lumens, creating an accessible "CSF-like" compartment for analysis of secreted factors, nutrients, and potential biomarkers.

Our Comprehensive Choroid Plexus Organoid Modeling Services

Creative Biolabs offers a suite of customizable services designed to accelerate your research and therapeutic development pipeline. We partner with you to design studies that meet your specific objectives:

1. Custom ChP Organoid Generation

We expertly generate high-quality, well-characterized ChP organoids from standard human iPSC lines, H9 cell line, or your own custom lines. We ensure robust development and validation of key ChP markers (TTR, ZO-1, CLIC6, etc.) and morphology, providing you with reliable models for downstream applications.

Fig.1 Characterization of ChP organoids.^2,3

2. Analysis of Organoid-Secreted "CSF"

Explore the brain's internal milieu without invasive procedures. We can carefully extract the fluid secreted within the organoid lumens (CSF) and perform detailed analyses (e.g., proteomics, metabolomics, specific factor quantification) to identify secreted proteins, potential biomarkers, or changes in response to treatment or disease states.

Fig.2 Clusterin (left) & IGF-2 (right) detected in ChP organoid CSF-like secretions.^2,3

Fig.3 Identification CSF proteins in CSF-like fluid by Mass Spectrometry.^2,3

3. Blood-CSF Barrier Modeling & Permeability Assessment

Need to know if your therapeutic candidate can reach the CNS? We utilize our functional ChP organoids to model the BCSFB, assessing barrier integrity and accurately measuring the permeability and transport kinetics of small molecules, biologics, or potential neurotoxins. Gain crucial data for predicting CNS drug delivery and safety.

4. Modeling Neurological Conditions & Pathogenesis

Gain deeper insights into diseases impacting the ChP and BCSFB. We can leverage ChP organoids to model aspects of:

• Infectious Diseases: Study pathogen (bacterial/viral) interactions, translocation across the BCSFB, and host responses (e.g., modeling meningitis).
• Neurodevelopmental & Neurodegenerative Disorders: Investigate potential ChP dysfunction or altered CSF composition linked to these conditions.
• Neuroinflammation & Toxicity: Assess the impact of inflammatory triggers or toxic exposures (e.g., environmental factors, radiation effects) on ChP health and barrier function.
• Choroid Plexus Tumorigenesis Mechanisms: Utilize genetically engineered iPSC-derived ChP organoids to investigate the fundamental mechanisms driving CPT initiation and growth. By manipulating key signaling pathways (such as the Wnt/ß-catenin pathway implicated in CPT development), we can help you dissect the molecular events underlying tumor formation and identify potential early intervention targets.

Focus Area: Advancing Choroid Plexus Tumor (CPT) Research

• Choroid plexus tumors (CPTs), though rare, pose significant therapeutic challenges. Creative Biolabs offers specialized Patient-Derived CPT Organoid (CPT-PDO) Models to accelerate progress in this critical area:
• CPT-PDO Generation & Characterization: We can establish CPT organoids directly from patient tumor samples (CPP, APP, CPC). These PDOs are meticulously characterized to ensure they faithfully represent the original tumor's histology, genomic landscape, and gene expression patterns.
• Preclinical Drug Efficacy Screening: Use our CPT-PDO platform to test the efficacy of novel anti-cancer agents, targeted therapies, or drug combinations directly on human tumor models. This provides highly relevant preclinical data to guide clinical strategy.
• CPT Biomarker & Target Discovery: Leverage CPT-PDOs and associated multi-omics analysis to identify new therapeutic targets, understand drug resistance mechanisms, and discover potential biomarkers specific to CPT subtypes.

Our Workflow

Partner with Creative Biolabs

Your research goals are our priority. We believe in a collaborative approach, working closely with you from project design through to data delivery. With Creative Biolabs, you gain access to:

• Cutting-edge, human-relevant ChP organoid models.
• Customizable study designs tailored to your needs.
• Rigorous quality control and validation.
• Expert scientific support and interpretation.
• Timely results to keep your projects moving forward.

Ready to explore the potential of choroid plexus organoids for your research? Contact Creative Biolabs today to discuss your project with our scientific team and discover how we can help you unlock new insights into CNS health and disease.

References

1. Bitanihirwe, Byron K Y et al. “Deconstructing the functional neuroanatomy of the choroid plexus: an ontogenetic perspective for studying neurodevelopmental and neuropsychiatric disorders.” Molecular psychiatry vol. 27,9 (2022): 3573-3582. doi:10.1038/s41380-022-01623-6
2. Chew, Leon H et al. “Methods to extract and analyze fluid from human pluripotent stem cell-derived choroid plexus organoids.” Frontiers in molecular neuroscience vol. 16 1243499. 29 Jan. 2024, doi:10.3389/fnmol.2023.1243499
3. Distributed under Open Access License CC BY 4.0 without modification.