Cystatin C EIA Kit, Human

Description

The Cystatin C EIA kit is designed to quantitatively measure human Cystatin C present in biological samples and tissue culture media. A human Cystatin C standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture the Cystatin C present. After a 60 minute incubation, the plate is washed and a peroxidase conjugated Cystatin C monoclonal antibody is added. The plate is again incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound Cystatin C Antibody Conjugate. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the Cystatin C in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

Species Reactivity

Human

Applications

Sandwich EIA (Enzyme Immunoassay)

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration

Sensitivity

0.058 ng/ml

Platform

Microplate

Detection Method

Colorimetric Assay

Sample Type

EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine

Assay Type

EIA Kits

Assay Time

2 Hours

Precision

Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cystatin C concentrations were: Sample 1- 7.72 ng/mL, 9.1% CV Sample 2- 5.08 ng/mL, 10.3% CV Sample 3- 4.42 ng/mL, 7.6% CV Sample 4- 0.88 ng/mL, 10.3% CV Inter Assay Precision: Four human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by three operators. The mean and precision of the calculated Cystatin C concentrations were: Sample 1- 7.79 ng/mL, 8.4% CV Sample 2- 5.48 ng/mL, 10.2% CV Sample 3- 4.97 ng/mL, 11.1% CV Sample 4- 1.04 ng/mL, 12.40% CV

Number Of Samples

40 samples in duplicate

Linear Range

0.156 - 10 ng/ml

Components

Clear 96 well Plate 1 Plate
Cystatin C Standard 60 µl
StressXpress® Cystatin C Conjugate 5 ml
Assay Buffer Concentrate 28 ml
Wash Buffer Concentrate 30 ml
TMB Substrate 5 ml
Stop Solution 5 ml
Plate Sealer 1 Each

Shipping

Blue Ice

Storage

4°C

Research Use Only

For research use only

Target

Cystatin C

Official Name

CST3

Full Name

cystatin 3

Alternative Names

CST3; ARMD11; HEL-S-2; cystatin C

Gene ID

1471(Human); 13010(Mouse); 25307(Rat)

Uniprot ID

P01034(Human); P21460(Mouse); P14841(Rat)

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