Excitotoxicity In Vitro Assay

Name: Excitotoxicity In Vitro Assay
SKU: NRZP-0922-ZP112
Rating: 5 (1 reviews)

[CAT#: NRZP-0922-ZP112]

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Assay Overview

Description

Brief exposure to glutamate in vitro can cause neuron death, which makes glutamate a useful tool for evaluating neuroprotective activity. Creative Biolabs' in vitro excitotoxicity assays provide the advantage of rapid analysis of molecular neuroprotective potential. In our analysis, we incubated primary rat neurons with the test compound 24 hours before L-glutamate injury. After 24 hours of exposure, we analyzed different parameters related to all stages of the cell death process. Some of the endpoints we analyzed were cell viability, LDH release, neurite outgrowth, or Caspase activation.

Assay Characteristics

Readout: Neurite Outgrowth, LDH Release, Caspase Activation, Cell Viability

Damage Induction: Glutamate

Type of Assay: Cell-based / Neuroprotection

Relevant Diseases

ALS; CNS

Research Areas

ALS; CNS

Cell Models Information

Cell Models Used

Rat Primary Neurons

Cell Models ID

NCL2110P251

Model Applications

In vitro Disease Models - Phenotypic Assays

Properties

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

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