Gamma Secretase Activity Assay Cell Line [Alzheimer's Disease (AD) Model]

[CAT#: NCL2110P232]

Species:: Human

Applications:: Cell Culture

Cell Types:: Other Cells

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Product Overview

Description

The γ-secretase activity assay cell line is an in vitro model of Alzheimer's disease used to identify inhibitors of γ-secretase activity. It contains a U2OS cell line stably expressing the green fluorescent APP-C99 construct, which contains a γ-secretase cleavage site. For testing, cells were incubated with test compounds for 24 hours to evaluate their effect on APP processing. After incubation, the nucleus can be stained with DAPI and the retained fluorescent APP vesicles can be detected by fluorescence using image analysis algorithms.

Features

Creative Biolabs offers this assay as a "stable cell line" in a cryopreserved vial. Each vial of the U2OS cell line stably expressing human tGFP-APP-C99 contains 3 million cells, and Creative Biolabs provides 2 vials of cells for each order. According to the cell culture manual, these cells can be guaranteed to pass more than 30 generations.

Neurological Disease Models

AD-related Cells

Cell Types

Other Cells

Applications

Cell Culture

Application Notes

Applications::
This model allows evaluation of compound libraries, candidates for gamma-secretase inhibitors, in living cells studying vesicle retention
Gamma Secretase Activity Assay provides a strategy to assess the effect of drugs on γ-secretase activity without having to be permeable.
This model allows the analysis of compound effects in space and time in a multi-parameter manner.

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.