Glutamate Dehydrogenase Inhibitor Screening Kit (Fluorometric), Mammalian

Description

Glutamate dehydrogenase (GDH) (1.4.1.2) is a hexamerase that catalyzes the reversible conversion of glutamate to α-ketoglutarate and ammonia, and at the same time reduces NAD + to NADH. Glutamate can be converted into α-ketoglutarate through GDH, and then flow into the TCA cycle, where it can be used to support oxidative phosphorylation, lipid production or supplement key intermediates (such as oxaloacetic acid). It is a key enzyme in the metabolic pathway of glutamine. Overexpression of GDH has been shown to promote cell proliferation, migration and invasion in a variety of tumors, such as colorectal cancer. The increased dependence on glutamine metabolism makes GDH a target for cancer therapeutic intervention. GDH inhibitors have been shown to be toxic to cancer cells in vitro. In our glutamate dehydrogenase inhibitor screening kit, glutamate is deaminated by NADH that produces GDH, which converts non-fluorescent probes into fluorescent products in the presence of an enzyme mixture, and the mixture passes fluorescence Detection is at Ex / Em = 535/587 nm. The kit provides a fast, simple, and sensitive plate-based detection method, and is also suitable for high-throughput screening of GDH inhibitors.
Screening of potential inhibitors of Glutamate Dehydrogenase

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Fluorescence at Ex/Em = 535/587 nm

Assay Type

kit

Components

• GDH Inhibitor
• GDH Enzyme
• GDH Probe
• GDH Assay Buffer
• GDH Developer
• GDH Substrate

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

Glutamate Dehydrogenase

Official Name

GLUD1

Full Name

Glutamate Dehydrogenase 1

Alternative Names

Glutamate Dehydrogenase 1; EC 1.4.1.3; GLUD; Epididymis Tissue Sperm Binding Protein Li 18mP; Glutamate Dehydrogenase 1, Mitochondrial; Glutamate Dehydrogenase (NAD(P)+)

Gene ID

2746(Human); 14661(Mouse); 24399(Rat)

Uniprot ID

P00367(Human); P26443(Mouse); P10860(Rat)