Creative Biolabs

Human PARK7 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP506]

Human PARK7 knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HEK 293T

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Description
This is a HEK293T cell transfected lysate in which Human PARK7 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HEK 293T
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Parkinson's Disease Signaling
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
Tissue Source
Kidney
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
PARK7
Official Name
PARK7
Full Name
Protein deglycase DJ-1
Alternative Names
PARK7; DJ-1; DJ1; HEL-S-67p; Parkinsonism associated deglycase; GATD2
Gene ID
11315(Human); 57320(Mouse); 117287(Rat)
Uniprot ID
Q99497(Human); Q99LX0(Mouse); O88767(Rat)
For Research Use Only. Not For Clinical Use.
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