Human Phosphotylinosital 3 kinase, PI3K ELISA Kit

[CAT#: NRZP-0722-ZP242]

For the quantitative determination of human phosphotylinosital 3 kinase (PI3K) concentrations in serum, cell lysates, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human PI3K.

Species Reactivity

Human

Marker

Phosphotylinosital 3 kinase,PI3K

Properites

Sensitivity

In most cases, the sensitivity is below 0.156 ng/ml.

Detection Method

Sandwich

Sample Type

serum, tissue homogenates and cell lysates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.625 ng/ml-40 ng/ml

Max Emission

450 nm

Target Details

Target

PIK3CA / p110 alpha

Official Name

PIK3CA

Full Name

phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha

Alternative Names

MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha

Gene ID

5290(Human)

Uniprot ID

P42336(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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