Rat Phosphotylinosital 3 kinase, PI3K ELISA Kit

Name: Rat Phosphotylinosital 3 kinase, PI3K ELISA Kit
SKU: NRZP-0722-ZP244
Rating: 5 (1 reviews)

[CAT#: NRZP-0722-ZP244]

For the quantitative determination of rat phosphotylinosital 3 kinase (PI3K) concentrations in serum, plasma, tissue homogenates, cell lysates.

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of rat PI3K.

Species Reactivity

Rat

Marker

Phosphotylinosital 3 kinase,PI3K

Properites

Sensitivity

In most cases, the sensitivity is below 3.9 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates, cell lysates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

15.6 pg/ml-1000 pg/ml

Max Emission

450 nm

Target Details

Target

PIK3CA / p110 alpha

Official Name

PIK3CA

Full Name

phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha

Alternative Names

MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha

Gene ID

170911(Rat)

Uniprot ID

A0A0G2K344(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

Publications