Creative Biolabs

Human RAB4A Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP244]

Human RAB4A knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

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Description
This is a HeLa cell transfected lysate in which Human RAB4A has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 1 bp insertion in exon 4.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neural Cell
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8, 12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Rab4
Official Name
RAB4A
Full Name
Ras-related protein Rab-4A
Alternative Names
RAB4A; HRES-1; HRES-1/RAB4; HRES1; RAB4; member RAS oncogene family
Gene ID
5867(Human); 19341(Mouse); 25532(Rat)
Uniprot ID
P20338(Human); P56371(Mouse); P05714(Rat)
For Research Use Only. Not For Clinical Use.
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