JNK Activity Assay Kit, KinaseSTAR, Mammalian
[CAT#: NK1120FY131]
Specifically detects JNK activity
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
The JNK activity immunoassay kit uses JNK specific antibodies to immunoprecipitate JNK from cell lysates. The recombinant c-Jun was then used as a substrate to determine the activity of JNK in the kinase reaction. The phosphorylation of c-Jun can be analyzed by Western blot analysis using phospho-c-Jun specific antibodies. The kit can specifically detect JNK, but not other kinase activities.
• The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. JNK-specific activity is then analyzed by determining the phosphorylation of c-Jun by Western blotting using a phospho-c-Jun specific antibody.
• The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. JNK-specific activity is then analyzed by determining the phosphorylation of c-Jun by Western blotting using a phospho-c-Jun specific antibody.
Species Reactivity
Mammalian
Application Notes
The assay utilizes a N-terminal c-Jun fusion protein bead to selectively "pull down" JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.
Relevant Diseases
Neurological Disorders
Properites
Detection Method
Western blot analysis using a phospho-c-Jun specific antibody at 1:1000 dilutions
Preparation and Storage
Shipping
Gel Pack
Storage
-20°C
Research Use Only
For research use only
Target Details
Publications
Publications (0)
Kits
Neural Antibodies
For Research Use Only. Not For Clinical Use.
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