Lenti of Mouse beta-site APP-cleaving enzyme 2 (Bace2) (NM_019517) ORF clone, mGFP Tagged

Description

This is a mouse BACE2 mGFP tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

BACE2

Species

Mouse

Insert Size (bp)

1542 bp

Tag

mGFP

Type

Expression Vector/ Viral Particle

Vector

pLenti

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGGCGCGCTGCTTCGAGCACTCTTGCTCCTGGTGCTGGCGCAGTGGCTCTTGAGTGCGGTCCCCGCGCTGGCCCCCGCGCCCTTCACGCTGCCCCTCCAAGTGGCCGGGGCCACGAACCACAGAGCCTCGGCTGTTCCCGGACTCGGGACCCCCGAGTTGCCCCGGGCCGATGGTCTGGCCCTCGCACTGGAGCCTGTCAGGGCTACTGCCAACTTCTTGGCTATGGTGGACAACCTTCAGGGGGACTCTGGCCGCGGCTACTACCTAGAGATGCTGATCGGGACCCCTCCGCAGAAGGTACAGATTCTTGTGGACACTGGAAGCAGTAACTTCGCTGTGGCAGGTGCCCCACACTCCTACATAGACACCTACTTTGACTCAGAGAGCTCCAGCACATACCACTCCAAGGGCTTTGATGTCACTGTGAAGTACACACAGGGAAGCTGGACTGGCTTTGTTGGTGAGGACCTTGTCACCATCCCAAAAGGCTTCAACAGCTCTTTCTTGGTCAATATTGCCACTATTTTCGAGTCTGAGAATTTCTTTTTGCCTGGTATTAAATGGAATGGAATCCTTGGACTTGCTTATGCTGCTTTGGCCAAGCCATCAAGCTCTCTGGAGACATTTTTTGATTCCCTGGTGGCCCAAGCAAAGATTCCAGACATTTTCTCCATGCAGATGTGCGGGGCTGGATTGCCAGTAGCTGGTTCTGGTACCAACGGAGGTAGTCTTGTCCTGGGTGGGATTGAACCAAGTTTGTATAAAGGAGATATCTGGTATACCCCAATTAAAGAGGAATGGTACTATCAAATAGAAATCCTGAAGTTGGAAATTGGAGGCCAGAACCTCAACCTGGACTGCAGAGAGTATAACGCAGACAAGGCCATTGTGGACAGTGGCACCACGCTCCTGCGCCTGCCCCAGAAGGTGTTTGATGCAGTGGTGGAAGCTGTGGCACGAACATCTCTGATTCCAGAGTTTTCTGATGGCTTCTGGACAGGGGCCCAGCTGGCATGCTGGACAAATTCTGAAACGCCATGGGCATATTTCCCTAAGATTTCTATCTACCTGAGAGATGAGAATGCCAGTCGCTCCTTCCGGATCACCATTCTCCCACAGCTCTACATTCAGCCCATGATGGGAGCTGGTTTCAATTATGAATGCTACCGTTTTGGTATCTCCTCTTCCACAAATGCGCTGGTGATTGGTGCGACCGTGATGGAAGGCTTCTACGTGGTCTTTGACAGAGCTCAGAGGAGGGTGGGCTTTGCAGTGAGTCCCTGTGCAGAGATTGAAGGTACCACAGTGTCTGAAATTTCTGGGCCCTTTTCCACGGAAGACATAGCCAGCAACTGTGTTCCAGCACAGGCTCTGAATGAGCCCATCTTGTGGATTGTGTCCTATGCCCTGATGAGTGTGTGTGGAGCCATTCTCCTGGTTCTGATCCTCCTCCTGCTGCTCCCGCTGCACTGCCGTCATGCCCCCCGAGACCCTGAGGTAGTTAACGATGAGTCCTCACTAGTCAGACATCGCTGGAAAACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA

Protein Sequence

MGALLRALLLLVLAQWLLSAVPALAPAPFTLPLQVAGATNHRASAVPGLGTPELPRADGLALALEPVRATANFLAMVDNLQGDSGRGYYLEMLIGTPPQKVQILVDTGSSNFAVAGAPHSYIDTYFDSESSSTYHSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNSSFLVNIATIFESENFFLPGIKWNGILGLAYAALAKPSSSLETFFDSLVAQAKIPDIFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQNLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARTSLIPEFSDGFWTGAQLACWTNSETPWAYFPKISIYLRDENASRSFRITILPQLYIQPMMGAGFNYECYRFGISSSTNALVIGATVMEGFYVVFDRAQRRVGFAVSPCAEIEGTTVSEISGPFSTEDIASNCVPAQALNEPILWIVSYALMSVCGAILLVLILLLLLPLHCRHAPRDPEVVNDESSLVRHRWKTRTRPLE - GFP Tag - V

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neurotransmission

Relevant Diseases

Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C

Research Use Only

For research use only

Target

BACE2

Official Name

BACE2

Full Name

Beta-secretase 2

Alternative Names

AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13; CEAP1; DRAP; beta-site APP-cleaving enzyme 2; beta-secretase 2

Gene ID

25825(Human); 56175(Mouse); 288227(Rat)

Uniprot ID

Q9Y5Z0(Human); Q9JL18(Mouse); Q6IE75(Rat)