Mouse interleukin 1 alpha (cDNA clone MGC:5780 IMAGE:3599550) (BC003727) ORF clone/Lentiviral Particle, Untagged

Description

This is a mouse IL1A untagged ORF clone expression plasmid or lentiviral particle, ready for transfecting into mammalian cells.

Gene/Insert Name

IL1A

Species

Mouse

Insert Size (bp)

813 bp

Tag

Tag Free

Type

Expression Vector/ Viral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Kanamycin (25 ug/mL)

Cell Selection

Neomycin

ORF Nucleotide Sequence

GCCACTGGTGTTCTCTTACAGAAATCAAGATGGCCAAAGTTCCTGACTTGTTTGAAGACCTAAAGAACTG TTACAGTGAAAATGAAGACTACAGTTCTGCCATTGACCATCTCTCTCTGAATCAGAAATCCTTCTATGAT GCAAGCTATGGCTCACTTCATGAGACTTGCACAGACCAGTTTGTATCTCTGAGAACCTCTGAAACGTCAA AGATGTCCAACTTCACCTTCAAGGAGAGCCGGGTGACAGTATCAGCAACGTCAAGCAACGGGAAGATTCT GAAGAAGAGACGGCTGAGTTTCAGTGAGACCTTCACTGAAGATGACCTGCAGTCCATAACCCATGATCTG GAAGAGACCATCCAACCCAGATCAGCACCTTACACCTACCAGAGTGATTTGAGATACAAACTGATGAAGC TCGTCAGGCAGAAGTTTGTCATGAATGATTCCCTCAACCAAACTATATATCAGGATGTGGACAAACACTA TCTCAGCACCACTTGGTTAAATGACCTGCAACAGGAAGTAAAATTTGACATGTATGCCTACTCGTCGGGA GGAGATGACTCTAAATATCCTGTTACTCTAAAAATCTCAGATTCACAACTGTTCGTGAGCGCTCAAGGAG AAGACCAGCCCGTGTTGCTGAAGGAGTTGCCAGAAACACCAAAACTCATCACAGGTAGTGAGACCGACCT CATTTTCTTCTGGAAAAGTATCAACTCTAAGAACTACTTCACATCAGCTGCTTATCCAGAGCTGTTTATT GCCACCAAAGAACAAAGTCGGGTGCACCTGGCACGGGGACTGCCCTCTATGACAGACTTCCAGATATCAT AAAAGCAGCCTTATTTCGGGAGTCTATTCACTTGGGAAGTGCTGACAGTCTGTATGTACCATGTACAGGA ACCTTCCTCACCCTGAGTCACTTGCACAGCATGTGCTGAGTCTCTGTAATTCTAAATGAATGTTTACCCT CTTTGTAAGAGAAGAGCAAACCCTAGTGGAGCCACCCCGACATATGATACTATCTGTTATTTTAAAGAGT ACCCTATAGTTTGCTCAGTACTAATCATTTTAATTACTATTCTGCATGGCATTCTTAGGAGGATCAAAAA GACTCTACACATATTACAGATGGGTTAACAAAGGGATAAAACAACTGAAAAGCACACTCAATGCATTTGG AATATAAATTCACAGACCAATCTCACTGTGCACCTTCGGCTTCAAAATGCCAGTTGAGTAGGATAAAGGT ATAAGAACTTAATGCTGTCATTTTCAAAAGGAAGGGGACAATAGCTACATCTTTCCTACCTCAGTGGGTT TTACTCCAGTGAGATCATTTGGATGAAATCCTCCTGTAACAGACCTCAAGAAGGAGACAGACTGTTGAAT GTTATTTTTAAGTTATTTTATATATGTATTTATAAATATATTTATGATAATTATATTATTTATGGAACAT CCTTAAATCCTCTGAGCTTGACAGGCATCCTCACAGCAGGATTTTCTAGGTGGTCAGTTAGATATAGTTT CCTCTAGAGCACCATGCTACAGACTTTACACTTTTTCCACAGCCACGAAGCTCTCTGTACATTCCTGTAC TTGGGAGCCCTTTCATCATGATCTTAATCTGTACTGTTTACTTTGTTCATCTAAAATGATAATTGAGTCA GTCTTTTTCCCTCCCATCCTTAAAGCTGTCTGGGTATTCTTACATCATTCAGTCTCACCTGTAACTAACA CCAACCATCTAAAGATGGAAAGAGCTTAACTGTGACAACCACATCACTGTTACCTGAAGTTTCTTTTCTA GAATGTAATCAGTGTTTCCCCTGGATTCCAATATTTTTTTCAAACCACAGTATCATGTAACTATCAACAA TAACAATCAACTCATTATTATTAATCATAATTAAATAAAACAAGTTTGAGCTGAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA

Restriction Sites

EcoRI-NotI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Cell; Metabolism

Relevant Diseases

Alzheimer's Disease; Neurological Disorders

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

IL-1α

Official Name

IL1A

Full Name

Interleukin 1 alpha

Alternative Names

IL1A; IL-1A; IL1; IL1-ALPHA; IL1F1; interleukin 1 alpha; IL-1 alpha

Gene ID

3552(Human)

Uniprot ID

P01583(Human)