Mouse Leptin ELISA Kit [Colorimetric], One-Step Assay

[CAT#: NPP2011ZP221]

Applications:: ELISA

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Product Overview

Description

The mouse leptin ELISA kit is a single-wash 90-minute sandwich ELISA for the quantitative determination of leptin protein in cell culture supernatant, plasma, serum and urine. It uses our proprietary SimpleStep ELISA® technology. Mouse leptin quantification, 33.6 pg/ml sensitivity.

Species Reactivity

Mouse

Applications

ELISA

Research Areas

Metabolism

Properites

Sensitivity

33.6 pg/ml

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Cell culture supernatant, Urine, Serum, Cit plasma

Assay Type

Sandwich (quantitative)

Assay Time

1h 30m

Assay Duration

One step assay

Linear Range

93.75 pg/ml - 6000 pg/ml

Components

Components 1 x 96 tests
10X Mouse Leptin Capture Antibody 1 x 600µl
10X Mouse Leptin Detector Antibody 1 x 600µl
10X Wash Buffer PT 1 x 20ml
Antibody Diluent CPI - HAMA Blocker 1 x 6ml
Mouse Leptin Lyophilized Recombinant Protein 2 vials
Plate Seals 1 unit
Sample Diluent NS 1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate 1 unit
Stop Solution 1 x 12ml
TMB Development Solution 1 x 12ml

Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only

Target Details

Target

Leptin

Official Name

LEP

Full Name

Leptin

Alternative Names

Leptin; Obese protein; OB; LEPD; Obesity factor; OBS

Gene ID

3952(Human);16846(Mouse)

Uniprot ID

P41159(Human);P41160(Mouse)

Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.