Rat Leptin ELISA Kit [Colorimetric], One-Step Assay

[CAT#: NPP2011ZP242]

Applications:: ELISA

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Product Overview

Description

The rat leptin ELISA kit is a single wash 90 min sandwich ELISA for the quantitative determination of leptin protein in cell culture supernatant, cit plasma, hep plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitative rat leptin, 24 pg/ml sensitivity.

Species Reactivity

Rat

Applications

ELISA

Research Areas

Metabolism

Properites

Sensitivity

24 pg/ml

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Cell culture supernatant, Serum, Hep Plasma, Cit plasma

Assay Type

Sandwich (quantitative)

Assay Time

1h 30m

Assay Duration

One step assay

Linear Range

47 pg/ml - 3000 pg/ml

Components

Components 1 x 96 tests
10X Rat Leptin Capture Antibody 1 x 600µl
10X Rat Leptin Detector Antibody 1 x 600µl
10X Wash Buffer PT 1 x 20ml
Antibody Diluent CPI - HAMA Blocker 1 x 6ml
Plate Seals 1 unit
Rat Leptin Lyophilized Recombinant Protein 2 vials
Sample Diluent NS 1 x 50ml
SimpleStep Pre-Coated 96-Well Microplate 1 unit
Stop Solution 1 x 12ml
TMB Development Solution 1 x 12ml

Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only

Target Details

Target

Leptin

Official Name

LEP

Full Name

Leptin

Alternative Names

Leptin; Obese protein; OB; LEPD; Obesity factor; OBS

Gene ID

3952(Human);16846(Mouse)

Uniprot ID

P41159(Human);P41160(Mouse)

Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.