Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein (NM_026880) ORF clone, Myc-DDK Tagged

Description

This is a mouse PINK1 Myc-DDK tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

PINK1

Species

Mouse

Insert Size (bp)

1740 bp

Tag

Myc-DDK

Type

Mouse Tagged ORF Clone

Vector

pLenti

E. coli Selection

Puromycin

Cell Selection

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGCGGTGCGACAGGCACTGGGCCGAGGCCTGCAGCTGGGTCGGGCGCTGCTGCTGCGCTTCGCGCCCAAGCCCGGCCCGCTGTTCGGCTGGGGGAAGCCCGGCCCCGCGGCGGCCTGGGGCCGCGGAGAGCGCCCAGGCCAGGTCGTAAGCCCCGGAGCACAGCCTCGGCCGGTCGGGCTCCCCCTCCCGGACCGCTACCGCTTCTTCCGCCAGTCGGTAGCTGGGCTGGCGGCGCGGATCCAGAGGCAGTTCATGGTGCGGGCCCGGGGCGGCGCAGGCCCTTGCGGCCGAGCGGTCTTCCTGGCCTTCGGGCTGGGGCTGGGGCTGATCGAGGAGAAGCAGGCGGAAGGCCGGAGGGCCGCCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACACAGAAAACCAAGCGCGTGTCTGACCCACTGGACACTCGATGCTGGCAGGGCTTCCGTCTGGAGGATTATCTGATAGGGCAAGCCATTGGCAAGGGTTGCAATGCCGCTGTGTATGAAGCCACCATGCCCACGCTGCCCCAGCACCTGGAAAAGGCCAAACACCTTGGCCTTATAGGAAAGGGCCCGGATGTCGTCCTGAAGGGAGCAGACGGGGAGCAGGCTCCAGGGACTCCCACCTTTCCCTTTGCCATCAAGATGATGTGGAATATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTAAGCAAAATGAGCCAGGAGCTGGTCCCGGCAAGCCGCGTGGCTTTGGCTGGAGAGTATGGAGCAGTTACTTACAGAAGATCCAGAGATGGTCCCAAGCAGCTTGCCCCACACCCTAACATCATCCGGGTTTTCCGCGCCTTCACCTCATCTGTGCCCCTCCTGCCGGGGGCCCTGGCTGACTATCCTGATATGCTGCCCCCACACTACTACCCAGAAGGCCTGGGCCACGGTCGCACACTGTTCCTCGTTATGAAGAACTACCCCTGTACCCTGCGCCAGTACCTTGAGGAGCAGACTCCCAGTTCTCGCCTGGCTACCATGATGACCTTGCAGTTGCTGGAGGGCGTGGACCATCTGGTTCAGCAGGGCATTGCCCATCGGGATCTCAAGTCCGACAACATCCTTGTGGAGTGGGACTCAGATGGCTGTCCCTGGCTAGTGATCTCAGACTTTGGCTGCTGCCTGGCTGACCAGCATGTTGGCCTGCGGCTGCCTTTCAACAGCTCCAGTGTAGAGCGTGGTGGCAATGGCTCCCTGATGGCCCCTGAGGTGTCCACAGCCCATTCTGGCCCCAGTGCGGTAATTGACTACAGCAAAGCCGATACCTGGGCTGTGGGGGCCATCGCCTATGAAATCTTTGGGCTTGCCAATCCCTTCTATGGCCAAGGCAGTGCCCACCTCGAGAGCCGCAGCTACCAGGAAGCTCAGCTGCCTGAGATGCCTGAGTCGGTGCCTCCAGAGGCAAGACGGCTGGTGAGGTCACTGCTCCAGCGAGAGGCCAGCAAGAGACCGTCTGCACGCTTAGCTGCAAATGTGCTGCACTTAAGCCTCTGGGGCGAGCATCTTCTAGCCCTGAAGAACCTGAAATTGGACAAGATGATCGCCTGGCTCTTGCAGCAGTCAGCAGCCACTCTGCTGGCTGACAGGCTGAGAGAGAAGAGCTGCGTGGAGACAAAGCTGCAGATGCTGTTTCTGGCTAACCTGGAGTGTGAGGCTCTCTGCCAGGCAGCCCTCCTCCTCTCTTCCTGGAGGGCAGCCCCAACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA

ORF Nucleotide Sequence

MAVRQALGRGLQLGRALLLRFAPKPGPLFGWGKPGPAAAWGRGERPGQVVSPGAQPRPVGLPLPDRYRFFRQSVAGLAARIQRQFMVRARGGAGPCGRAVFLAFGLGLGLIEEKQAEGRRAASACQEIQAIFTQKTKRVSDPLDTRCWQGFRLEDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHLGLIGKGPDVVLKGADGEQAPGTPTFPFAIKMMWNISAGSSSEAILSKMSQELVPASRVALAGEYGAVTYRRSRDGPKQLAPHPNIIRVFRAFTSSVPLLPGALADYPDMLPPHYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLATMMTLQLLEGVDHLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCLADQHVGLRLPFNSSSVERGGNGSLMAPEVSTAHSGPSAVIDYSKADTWAVGAIAYEIFGLANPFYGQGSAHLESRSYQEAQLPEMPESVPPEARRLVRSLLQREASKRPSARLAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATLLADRLREKSCVETKLQMLFLANLECEALCQAALLLSSWRAAPTRTRPLE - GFP Tag - V

Protein Sequence

SgfI-MluI

Application Notes

Ion-exchange column purified

Purification

Neural Diseases

Research Areas

Metabolism

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

PINK1

Official Name

PINK1

Full Name

PTEN induced putative kinase 1

Alternative Names

BRPK;
FLJ27236;
mitochondrial;
PARK 6;
PARK6;
Phosphatase and Tensin Homolog;
PINK 1;
PINK1;

Gene ID

65018(Human)

Uniprot ID

Q9BXM7(Human)

1. Espinoza-Derout, J., Shao, X. M., Bankole, E., Hasan, K. M., Mtume, N., Liu, Y., ... & Friedman, T. C. (2019). Hepatic DNA damage induced by electronic cigarette exposure Is associated with the modulation of NAD+/PARP1/SIRT1 axis. Frontiers in Endocrinology, 10, 320.
2. under Open Access license CC BY 4.0.