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Creative Biolabs

Neural Tissue Dissociation Kit (P) - Postnatal Neurons

[CAT#: NRZP-0622-ZP15]

Applications:
Tissue Dissociation

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Product Overview

Description

The Neural Tissue Dissociation Kit was developed for gentle and efficient generation of single-cell suspensions from neural tissue in the neonatal brain (≤ P7). The Neural Tissue Isolation Kit (P) contains papain and the Neural Tissue Isolation Kit (T) contains trypsin.

Applications

Tissue Dissociation

Principle

Neural tissue from neonatal brains (animal age ≤P7) can be dissociated into single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular matrix, thereby maintaining the structural integrity of the tissue. Neural tissue was enzymatically digested using kit components. Cells should be processed immediately for downstream applications such as cell isolation, cell culture, cellular or molecular analysis.

Size

50 preparations
Properites

Components

2.5 mL of Enzyme P
2×50 mL of Buffer X (sterile)
1.5 mL of Buffer Y (sterile)
1 vial of Enzyme A (lyophilized powder)
1 mL of Buffer A
Publications

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For Research Use Only. Not For Clinical Use.
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