Mouse Anti-Human EGFR Monoclonal Antibody (CBP1587)

Product Overview

Description

The mouse anti-human EGF receptor recognizes the epidermal growth factor (EGF) receptor, a 150 kDa transmembrane receptor, also known as ERBB1. The EGF receptor is a member of the ERBB family of four structurally related receptor tyrosine kinases. The binding of one of many EGF receptor ligands (EGF, Amphiregulin, TGFα, Epiregulin) can cause receptor dimerization. This leads to the downstream activation of multiple signaling pathways including MAPK/ERK, PI3K/Akt and PLCγ/PKC. The overexpression of EGF receptor promotes gene amplification and mutation, leading to cell proliferation, survival, invasion, metastasis and tumor-induced neovascularization.

Immunogen

E. coli derived recombinant protein corresponding to aa 363-499 of the human EGF receptor.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP1587

Applications

ELISA; WB

Application Notes

WB (1/1000)

Research Areas

Growth and Development

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein G from ascites.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

EGFR

Official Name

EGFR

Full Name

Epidermal Growth Factor Receptor

Alternative Names

Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian);

Gene ID

1956(Human); 13649(Mouse); 24329(Rat)

Uniprot ID

P00533(Human); Q01279(Mouse)

References

1. Ciardiello, F. & Tortora, G. (2008) EGFR antagonists in cancer treatment. 2. Prenzel, N. et al. (2001) The epidermal growth factor receptor family as a central element for cellular signal transduction and diversification.

Publications