Mouse Anti-THETM alpha Tubulin Monoclonal Antibody (CBP2125)

Product Overview

Description

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to α-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.

Immunogen

A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b,κ

Clone Number

CBP2125

Applications

ELISA; WB

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/mlImmunofluorescent staining: 1 µg/ml
Other Applications: user-optimized

Conjugation

Unconjugated

Product Properties

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification

Protein A affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.