Mouse Anti-THETM alpha Tubulin Monoclonal Antibody [HRP] (CBP2126)

Product Overview

Description

THETM α Tubulin Antibody [HRP], mAb, Mouse is HRP conjugated to THETM α Tubulin Antibody [HRP], mAb, Mouse (Cat. No. A01410) purified from mouse ascites by protein A affinity column. The product is a specific and useful tool in studying proper interpretation of western blots.

Immunogen

KLH-coupled synthetic peptide within residues 400-450 of human α-tubulin (Swiss Prot: P68363).

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

Mouse IgG2b, κ

Clone Number

CBP2126

Applications

ELISA; WB

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-0.5 µg/ml
Other Applications: user-optimized

Conjugation

Peroxidase (Horseradish)

Product Properties

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.