Mouse Anti-IL-1 beta Monoclonal Antibody (CBP2138)

Product Overview

Description

Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Immunogen

Human IL-1β full length recombinant protein

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

Mouse IgG1,κ

Clone Number

CBP2138

Applications

ELISA

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml.

Conjugation

Unconjugated

Product Properties

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification

Protein A affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.