Mouse Anti-Human Cathepsin B Monoclonal Antibody (CBP2183)

Description

Cathepsin B (CTSB) is a lysosomal cysteine protease. While most cathepsins are exclusively endopeptidases, CTSB exhibits both carboxypeptidase and endopeptidase activities. The optimal pH for CTSB activity is between four and six. Cystatin C has been identified as an endogenous CTSB inhibitor. High CTSB protein levels and activity have been found in many tumors including breast, cervix, colon, stomach, glioma, lung, and thyroid. CTSB can be secreted by tumor cells and is associated with the cell membrane of these cells. Membrane associated CTSB promotes extracellular matrix (ECM) degradation, which contributes to cancer motility and invasion. Many ECM proteins, including laminin, fibronectin, and collagen IV, are substrates of CTSB. CTSB can also activate pro-uPA/PLAU. Activated uPA promotes ECM digestion through serine protease plasminogen. It has been shown that the inhibition of CTSB can limit bone metastasis in breast cancer, which makes it an important anti-cancer drug target. CTSB has been proposed as a new drug target for Alzheimer's disease because of its involvement in the production of neurotoxic β-amyloid (Aβ) peptides. The inhibition of CTSB can reduce the brain Aβ peptides and improve memory that was determined from a mouse model of Alzheimer's disease. CTSB also plays significant roles in immune responses including both T and B cell apoptosis and Th1/Th2 polarization. CTSB is implicated in other pathological conditions including cardiovascular disease, multiple sclerosis, and arthritis. CTSB may also have a role in autophagy, adipogenesis, and cholesterol absorption in the intestine.

Immunogen

Partial human cathepsin B recombinant protein (18-339 a.a.) expressed in E. coli.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2183

Applications

WB

Application Notes

WB (0.5-2.5 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Cathepsin B

Official Name

CTSB

Full Name

Cathepsin B

Alternative Names

CTSB; CPSB; APP secretase; APPS; Cathepsin B1

Gene ID

1508 (Human); 13030 (Mouse); 64529 (Rat)

Uniprot ID

P07858 (Human); P10605 (Mouse); P00787 (Rat)

1. Mohamed MM, Sloane BF. 2006. Nat. Rev. Cancer 6:764.
2. Wang C, et al. 2012. J. Biol. Chem. 47:39834.
3. Grzonka Z, et al. 2001. Acta Biochim. Pol. 48:1.
4. Liu J, et al. 2006. FEBS Letters 580:245.
5. Brix K, et al. 2008. Biochimie. 90:194.
6. Turk V, et al. 2012. Biochim. Biophys. Acta. 1824:68.