Mouse Anti-Human Lamin A Monoclonal Antibody (CBP2334)

Product Overview

Description

Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues and is expressed at high levels in some tumor tissues. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been linked to cryptogenic cirrhosis. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2), and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3γ, 14-3-3ζ, 14-3-3σ, 14-3-3β, 14-3-3η, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others.

Immunogen

Synthetic peptide of human keratin 18 (CSETNDTKVLRH-COOH).

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2334

Applications

WB; ICC; IHC-P

Application Notes

WB (0.125-0.5 µg/mL); ICC (1.0-5.0 μg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

LMNA

Official Name

LMNA

Full Name

Lamin A/C

Alternative Names

LMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; PRO1; lamin A/C; MADA

Gene ID

4000 (Human); 16905 (Mouse)

Uniprot ID

P02545 (Human); P48678 (Mouse)

References

1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. USA 100:6063.
2. Kulesh DA, Oshima RG. 1989. Genomics 4:339.
3. Steinert PM, Roop DR. 1988. Ann. Rev. Biochem. 57:593.
4. Izawa I, et al. 2000. J. Biol. Chem. 275:34521.
5. Pankov R, et al. 1994. Proc. Natl. Acad. Sci. USA 91:873.

Publications