Mouse Anti-Human SIRT5 Monoclonal Antibody (CBP2379)

Product Overview

Description

The seven mammalian Sirtuin isoforms are assigned to different Sirtuin classes on the basis of sequence homology. The mitochondrial SIRT5 belongs to class III. In the mitochondrial matrix, Sirt5 deacetylates and activates carbamoyl phosphate synthetase 1 (CPS1), which is increased when amino acid metabolism is upregulated during fasting. More importantly, SIRT5 regulates lysine succinylation and malonylation as evolutionarily conserved post-translation modifications. SIRT5 was proved to regulate many metabolic pathways, with fatty acid beta-oxidation and ketone body production as two pathways strongly enriched in lysine succinylation. Recently, Zhang et al demonstrated that SIRT5 was targeted to protein complexes on the inner mitochondrial membrane via affinity for cardiolipin in order to promote respiratory chain function.

Immunogen

Full length protein expressed in 293T cell transfected with human SIRT5 expression vector.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b, κ

Clone Number

CBP2379

Applications

Application Notes

WB (0.2-2 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

SIRT5

Official Name

SIRT5

Full Name

Sirtuin (silent mating type information regulation 2 homolog) 5

Alternative Names

SIRT5; SIR2L5; sirtuin 5

Gene ID

23408 (Human); 68346 (Mouse)

Uniprot ID

Q9NXA8 (Human); Q8K2C6 (Mouse)

References

1. Wegner M. 1999. Nucleic Acids Res. 27: 1409.
2. Gertz M, et al. 2016. Cell. Mol. Life Sci. 73:2871.
3. Osborne B, et al. 2016. Free Radic. Biol. Med. 100:164.
4. Nakagawa T, et al. 2009. Cell 137:560.
5. Peng, et al. 2011. Mol. Cell. Proteomics 10:M111.012658.
6. Guan KL and Xiong Y. 2010. Trends Biochem. Sci. 36: 108.
7. Rardin MJ, et al. 2013. Cell. Metab. 18:920.
8. Zhang Y, et al. 2017. J. Biol. Chem. M117.785022.

Publications