Mouse Anti-CFL1 Monoclonal Antibody (CBP2684)

Product Overview

Description

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Expression Host

Mammalian cells

Immunogen

Cofilin fusion protein Ag18044

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2684

Applications

IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Metabolism

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

807 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Cofilin 1

Official Name

CFL1

Full Name

Cofilin 1

Alternative Names

18 kDa phosphoprotein; CFL; CFL1; Cofilin; Cofilin 1; cofilin 1 (non muscle); Cofilin; non muscle isoform; p18

Gene ID

1072(Human); 12631(Mouse); 29271(Rat)

Uniprot ID

P23528(Human); P18760(Mouse); P45592(Rat)

References

1. Sivadasan, R., Hornburg, D., Drepper, C., Frank, N., Jablonka, S., Hansel, A.,... & Ince, P. G. (2016). C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons. Nature neuroscience, 19(12), 1610-1618. 2. Sheng, N., Tan, G., You, W., Chen, H., Gong, J., Chen, D.,... & Wang, Z. (2017). MiR-145 inhibits human colorectal cancer cell migration and invasion via PAK4-dependent pathway. Cancer medicine, 6(6), 1331-1340. 3. Cui, K., Ruan, Y., Wang, T., Rao, K., Chen, Z., Wang, S., & Liu, J. (2017). FTY720 supplementation partially improves erectile dysfunction in rats with streptozotocin-induced type 1 diabetes through inhibition of endothelial dysfunction and corporal fibrosis. The Journal of Sexual Medicine, 14(3), 323-335.

Publications