Mouse Anti-TJP1 Monoclonal Antibody (CBP2778)

Product Overview

Description

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation.

Expression Host

Mammalian cells

Immunogen

ZO-1 fusion protein Ag16454

Species Reactivity

Human; Dog

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2778

Applications

IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Neurogenesis

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

TJP1

Official Name

TJP1

Full Name

Tight junction protein 1

Gene ID

7082(Human); 21872(Mouse); 292994(Rat)

Uniprot ID

Q07157(Human); P39447(Mouse)

References

1. Zhou, F., Jiang, Z., Yang, B., & Hu, Z. (2019). Magnolol exhibits anti-inflammatory and neuroprotective effects in a rat model of intracerebral haemorrhage. Brain, behavior, and immunity, 77, 161-167. 2. Zhai, J., Geng, X., Ding, T., Li, J., Tang, J., Chen, D.,... & Wang, Q. (2019). An increase of estrogen receptor α protein level regulates BDE-209-mediated blood-testis barrier disruption during spermatogenesis in F1 mice. Environmental Science and Pollution Research, 26(5), 4801-4820. 3. Zhang, X., Han, S., Zhou, H., Cai, L., Li, J., Liu, N.,... & Miao, Y. (2019). TIMM50 promotes tumor progression via ERK signaling and predicts poor prognosis of non-small cell lung cancer patients. Molecular carcinogenesis, 58(5), 767-776.

Publications