Mouse Anti-Human Complement C4b Monoclonal Antibody (CBP4302)

Description

The complement system is a part of eukaryotic innate defense system that enhances the clearance of exogenous microbes and damaged cells by antibodies and phagocytic cells. In vivo, the complement system is activated by three pathways: the classical complement pathway, the alternative complement pathway, and the lectin pathway. Complement C5 is a 1676 amino acid precursor protein synthesized in the liver. All three complement activation pathways culminate in the cleavage of C5, which produces two disulfide linked glycoproteins C5α and C5β. C5α is further cleaved by C5 convertase into C5a and C5α', the latter binds to C5β to form C5b, which in turn sequentially recruits C6-C9 to form the membrane attack complex (MAC). MAC destroys pathogens by forming a lytic pore in their membrane. Mutations affecting the Complement C5 gene lead to complement component 5 deficiency where patients demonstrate an increased propensity to develop severe recurrent infections.

Immunogen

KLH conjugated synthetic peptide corresponding to amino acids 428-454 of human complement C5 protein

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgM, κ

Clone Number

CBP4302

Applications

WB; IHC-P

Application Notes

WB ( 2.0-10 µg/mL); IHC-P (2.0 - 10 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Complement C4b

Official Name

C4B

Full Name

Complement component 4B

Alternative Names

C4B; C4B1; C4B12; C4B2; C4B3; C4B5; C4BD; C4B_2; C4F; CH; CO4; CPAMD3; Complement component 4B; complement component 4B (Chido blood group); complement C4B (Chido blood group)

Gene ID

Uniprot ID

1. Vlaicu SI, et al. 2019. Front Immunol. 10:1054
2. Fishelson Z, et al. 2019. Front Immunol. 10:752
3. Bergseth G, et al. 2013. Mol Immunol. 56(3):232