Hamster Anti-Mouse MCP-1 Monoclonal Antibody (CBP4349)

Product Overview

Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 4E2/MCP antibody reacts with mouse MCP-1. This antibody does not react with human MCP-1 and related mouse beta chemokines, TCA3 and MIP-1beta. The 4E2/MCP antibody can not neutralize the bioactivity of natural or recombinant MCP-1.

Immunogen

CHO-expressed, recombinant mouse MCP-1

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Hamster

Isotype

IgG

Clone Number

CBP4349

Applications

ELISA; ELISPOT

Application Notes

ELISA (1-4 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Biotin

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

MCP-1

Official Name

CCL-2

Full Name

Chemokine (C-C motif) ligand 2

Alternative Names

CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF; C-C motif chemokine ligand 2

Gene ID

6437

Uniprot ID

P13500

References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271.
3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.

Publications