NeuroMab™ Rabbit Anti-GAD65/GAD67 Monoclonal Antibody (CBP984)

Immunogen

Recombinant fragment Within Mouse GAD65 + GAD67 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: P48318

Species Reactivity

Mouse; Rat; Human

Clonality

Monoclonal

Host Species

Rabbit

Isotype

IgG

Clone Number

CBP984

Applications

IP; IHC-P; IHC-Fr; WB; ICC; IF

Application Notes

IP (1:40); IHC-P (1:1000); IHC-Fr (1:2500); WB (1:1000); ICC/IF (1:100)

Research Areas

Neural Cell

Marker

Soma

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1036

Form

Liquid

Formulation

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

Purification

Protein A purified

Purity

>95%

Endotoxin Level

Low Endotoxin

Shipping

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Storage

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes: avoid repeated freeze-thaw cycles.

Research Use Only

NAB-0720-Z3848 is designed for research use only, not for diagnostic or therapeutic use.

Target

GAD65; GAD67

Official Name

GAD65; GAD67

Full Name

glutamic acid decarboxylase (GAD)

Alternative Names

Glutamate decarboxylase or glutamate decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate into GABA and CO 2. GAD uses PLP as a cofactor. The reaction proceeds as follows:
HOOC-CH2-CH2-CH(NH2)-COOH→CO2 + HOOC-CH2-CH2-CH2NH2

In mammals; GAD exists in two isoforms with molecular weights of 67 and 65 kDa (GAD67 and GAD65); which are encoded by two different genes on different chromosomes (GAD1 and GAD2 genes; which are human 2 and Chromosome 10). GAD67 and GAD65 are expressed in the brain using GABA as a neurotransmitter; and are also expressed in insulin-producing beta cells of the pancreas; depending on the species. These two enzymes together maintain the main physiological supply of mammalian GABA; although it may also be caused by the enteric nervous system; brain; and other places through the action of diamines Synthesized from putrescine. Oxidase and aldehyde dehydrogenase 1a1.

Several truncated transcripts and peptides of GAD67 can be detected in the developing brain; but their function (if any) is unknown.

Gene ID

2572(GAD2); 2571(GAD1)

Uniprot ID

Q05329(GAD2)