Rat PPIB siRNA

[CAT#: NV-2106-P21]

Target:: PPIB

Type:: siRNA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

PPIB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Species

Rat

Type

siRNA

Applications

RNAi

Properties

Appearance

Lyophilized

Purity

> 97%

Shipping

Shipped at 4 °C.

Handling Advice

1. Transfect with 100 nM siRNA 48 to 72 hours prior to cell lysis.
2. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube.
3. Resuspend the siRNA oligos to an appropriate concentration with DEPC water.

Storage

Store at -20 °C for up to one year.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.