RBP4 ELISA Kit, Mammalian

Description

Retinol binding protein (RBP) 4 is the only specific transporter of vitamin A in the circulation. Its function is to transport vitamins to target tissues. In obesity and type 2 diabetes, the expression of Glut4 is significantly impaired in adipocytes. Glucose transport through Glut4 is the rate-limiting step for muscle and adipose tissue to use glucose. The lack of specific Gluts in adipocytes leads to a significant increase in RBP4, causing systemic insulin resistance. The reduction of RBP4 can improve insulin resistance, which proves that RBP4 is regulating insulin. It has a new role. Insulin action and RBP4 are recorded as fat cell-derived hormones. RBP4 (human) ELISA kit is used to quantitatively determine human RBP4 in biological fluids in vitro. The assay is a sandwich ELISA that can use a 96-well microtiter. HRP conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzene added with pyridine) to produce a color intensity proportional to the concentration of RBP4 in the sample. This ELISA is suitable for natural and recombinant The measurement of human RBP4 is specific. It does not interact with mouse RBP4, rat RBP4, human adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-33, human GPX3, human progranulin, Human FABP4, human ANGPTL3, human ANGPTL4, human ANG1 cross-reaction, human ANG2, human Nampt, mouse Nampt. The measurement range is 0.39 - 25 ng RBP4/ml, and the detection limit is 380 pg/ml (based on combining the two standards The difference is added to the average of (50) zero standards).

This ELISA kit is used for this assay detects human RBP4 in the range of 0.39 - 25 ng RBP4/ml and a detection limit of 380 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Components

• Pre-coated Microtiter Plate
• Wash Buffer (10X)
• Diluent (5X)
• Detection Antibody
• Detector 100X (Hrp conjugated anti-IgG)
• Mouse RBP4 Standard (lyophilized, 24 ng)
• Mouse RBP4 QC Sample (lyophilized)
• Substrate Solution I (TMB)
• Substrate Solution II (Peroxidase)
• Stop Solution
• Plate Sealers

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only

Target

Retinal Protein 4

Official Name

RBP4

Full Name

Retinol binding protein 4

Alternative Names

RBP4; MCOPCB10; RDCCAS; retinol binding protein 4

Gene ID

19662(Mouse); 25703(Rat)

Uniprot ID

Q00724(Mouse); P04916(Rat)