RBP4ELISA Kit, Mammalian

Description

Retinol-binding protein (RBP) 4 is the only specific transporter of vitamin A in the circulation, and its function is to transport vitamins to target tissues. In obesity and type 2 diabetes, the expression of Glut4 in adipocytes is significantly impaired. Glucose transport via Glut4 is the rate-limiting step for muscle and adipose tissue to use glucose. Adipose-specific loss of fat cells leads to a significant increase in mouse RBP4, causing systemic insulin resistance, while the decrease in RBP4 improves insulin resistance. This confirmed the new role of RBP4 in regulating the action of insulin, and RBP4 was recorded as a fat cell-derived hormone. RBP4 (mouse/rat) ELISA kit is used for in vitro quantitative determination of mouse or rat RBP4 in serum, urine and cell culture supernatant. The assay is a sandwich ELISA, which utilizes a 96-well microtiter plate, which is pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. Add HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) to produce a color intensity proportional to the concentration of RBP4 in the sample. This ELISA is specific for the measurement of natural and recombinant mouse and rat RBP4. It does not interact with human RBP4, mouse adiponectin, mouse resistin, mouse Nampt, rat adiponectin, rat resistin, rat Nampt, mouse RELM-α, mouse RELM-β, human Leptin, rat RELM-α, mouse 41BBL, mouse cross-reactive CD137, mouse Tim3, human TNF-α. The measurement range is 0.188 - 12 ng RBP4/ml, and the detection limit is 60 pg/ml (based on adding two standard deviations to the average value of (50) zero standards).
This ELISA kit is used for this assay detects mouse/rat RBP4 in the range of 0.188 - 12 ng RBP4/ml and a detection limit of 60 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Detection Range

0.188 - 12 ng

Components

• Pre-coated Microtiter Plate
• Wash Buffer (10X)
• Diluent (5X)
• Detection Antibody
• Detector 100X (Hrp conjugated anti-IgG)
• Mouse RBP4 Standard (lyophilized, 24 ng)
• Mouse RBP4 QC Sample (lyophilized)
• Substrate Solution I (TMB)
• Substrate Solution II (Peroxidase)
• Stop Solution
• Plate Sealers

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only

Target

Retinal Protein 4

Official Name

RBP4

Full Name

Retinol binding protein 4

Alternative Names

RBP4; MCOPCB10; RDCCAS; retinol binding protein 4

Gene ID

19662(Mouse); 25703(Rat)

Uniprot ID

Q00724(Mouse); P04916(Rat)