RBP4 Competitive ELISA Kit, Mammalian

Description

Retinol-binding protein (RBP) 4 is the only specific transporter of vitamin A in the circulation, and its function is to transport vitamins to target tissues. In obesity and type 2 diabetes, the expression of Glut4 in adipocytes is significantly impaired. Glucose transport via Glut4 is the rate-limiting step for muscle and adipose tissue to use glucose. Adipose-specific loss of fat cells leads to a significant increase in mouse RBP4, causing systemic insulin resistance, while the decrease in RBP4 improves insulin resistance. This confirmed the new role of RBP4 in regulating the action of insulin, and RBP4 was recorded as a fat cell-derived hormone. The RBP4 (human) ELISA kit is used for the in vitro quantitative determination of human RBP4 in serum, urine and cell culture supernatant. The assay is a competitive ELISA, which utilizes a 96-well microtiter plate pre-coated with human RBP4. The purified polyclonal recognizing natural human RBP4 reacts with a series of predetermined recombinant human RBP4 standard proteins or test samples in a plate coated with human RBP4. Plot its relative reactivity with standard proteins. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not interact with mouse RBP4, rat RBP4, human adiponectin, human adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-23, human IL-33, human GPX3, Human Nampt, human ANG1, human ANG2, human ANGPTL3, human ANGPTL4, human ANGPTL6, human FABP4, human RELM-β, rat RELM-α, mouse Nampt, human PAI-1. The measurement range is 0.001 - 5 µg RBP4 / ml, and the detection limit is 1 ng / ml (based on adding two standard deviations to the average value of (50) zero standards).
This ELISA kit is used for this assay detects human RBP4 in the range of 0.001 - 5 µg RBP4/ml and a detection limit of 1 ng/ml (based on adding two standard deviations to the mean value of the (50) zero standards).

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Detection Range

0.001 - 5 µg RBP4/ml

Components

• Pre-coated Microtiter Plate
• Wash Buffer (10X)
• Diluent (5X)
• Detection Antibody
• Detector 100X (Hrp conjugated anti-IgG)
• Mouse RBP4 Standard (lyophilized, 24 ng)
• Mouse RBP4 QC Sample (lyophilized)
• Substrate Solution I (TMB)
• Substrate Solution II (Peroxidase)
• Stop Solution
• Plate Sealers

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only

Target

Retinal Protein 4

Official Name

RBP4

Full Name

Retinol binding protein 4

Alternative Names

RBP4; MCOPCB10; RDCCAS; retinol binding protein 4

Gene ID

19662(Mouse); 25703(Rat)

Uniprot ID

Q00724(Mouse); P04916(Rat)