App Rat amyloid beta (A4) precursor protein (NM_019288) ORF clone/Lentiviral Particle, Myc-DDK Tagged

Description

This is a rat APP Myc-DDK tagged ORF clone expression plasmid or lentiviral particle, ready for transfecting into mammalian cells.

Gene/Insert Name

APP

Species

Rat

Insert Size (bp)

2310 bp

Tag

Myc-DDK

Type

Expression Vector/ Viral Particle

Vector

pCMV6

Vector Type

CMV

Virus Type

CMV

E. coli Selection

Kanamycin (25 ug/mL)

Cell Selection

Neomycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGCTGCCCAGCCTGGCACTGCTCCTGCTGGCCGCCTGGACGGTTCGGGCTCTGGAGGTGCCCACTGATGGCAATGCCGGTCTGCTGGCAGAACCCCAGATCGCCATGTTCTGTGGTAAACTCAACATGCACATGAATGTGCAGAATGGAAAATGGGAGTCAGACCCATCAGGGACCAAAACCTGCATTGGCACCAAGGAGGGAATCCTGCAGTACTGCCAAGAGGTCTACCCTGAACTGCAGATCACAAACGTGGTGGAAGCCAACCAGCCAGTGACCATCCAGAACTGGTGCAAGCGGGGCCGCAAGCAGTGCAAGACGCACACCCACATCGTGATTCCTTACCGGTGCCTAGTTGGTGAGTTTGTAAGCGATGCCCTTCTCGTGCCCGACAAGTGCAAGTTTCTACACCAGGAGCGGATGGACGTTTGTGAGACCCATCTTCACTGGCATACTGTTGCCAAAGAGACATGCAGTGAGAAGAGCACTAACTTGCACGACTATGGCATGCTGCTGCCCTGTGGCATCGACAAGTTCCGAGGGGTCGAGTTCGTGTGCTGCCCGTTGGCGGAGGAGAGCGACAGCATCGATTCTGCGGACGCAGAGGAGGACGACTCCGATGTCTGGTGGGGTGGAGCGGACACAGACTATGCTGATGGCGGTGAAGACAAAGTCGTAGAAGTAGCCGAAGAGGAGGAAGTGGCCGATGTTGAGGAAGAAGAAGCTGAGGATGACGAGGATGTGGAGGATGGGGATGAGGTGGAGGAGGAGGCCGAGGAGCCCTACGAAGAGGCCACAGAGAGAACAACCAGCATTGCCACCACTACCACAACTACCACTGAGTCTGTGGAGGAGGTAGTCCGAGAGGTGTGCTCTGAACAAGCTGAGACCGGGCCATGCCGTGCAATGATCTCCCGCTGGTACTTTGATGTCACTGAAGGAAAGTGCGCCCCATTCTTTTACGGCGGATGTGGCGGCAACAGGAACAACTTTGACACTGAAGAGTACTGCATGGCGGTGTGTGGCAGCGTGTCATCCCAAAGTTTACTCAAGACTACCAGTGAACCTCTTCCCCAAGATCCGGTTAAACTTCCCACGACGGCAGCCAGCACCCCTGACGCAGTCGACAAGTACCTGGAGACCCCCGGAGATGAGAACGAGCACGCCCATTTCCAGAAAGCCAAAGAGAGGTTGGAAGCCAAGCACCGAGAGAGAATGTCCCAGGTCATGAGAGAATGGGAGGAGGCAGAACGTCAAGCCAAGAATTTGCCCAAAGCTGACAAGAAGGCCGTTATCCAGCATTTCCAGGAGAAAGTGGAATCTCTGGAACAGGAAGCAGCCAACGAGAGGCAGCAGCTTGTAGAGACACACATGGCCAGAGTTGAAGCCATGCTCAATGATCGCCGTCGCCTGGCCCTCGAGAATTACATCACCGCACTGCAGGCGGTGCCTCCAAGGCCTCATCATGTGTTCAACATGCTGAAGAAGTACGTCCGTGCAGAGCAGAAGGACAGACAGCACACCCTAAAGCATTTTGAACATGTGCGCATGGTGGACCCCAAGAAAGCTGCTCAGATCCGGTCCCAGGTTATGACACACCTCCGTGTGATCTACGAGCGCATGAACCAGTCTCTGTCCCTGCTCTACAACGTCCCTGCCGTGGCTGAGGAGATTCAAGATGAAGTTGACGAGCTGCTTCAGAAGGAGCAGAACTACTCCGACGACGTCTTAGCCAACATGATCAGTGAACCCAGAATCAGTTACGGCAACGATGCTCTCATGCCTTCTTTGACTGAAACGAAGACCACTGTGGAGCTCCTTCCCGTGAATGGCGAATTCAGCCTGGATGATCTCCAACCGTGGCATCCTTTTGGGGTGGACTCTGTGCCAGCCAATACAGAAAATGAAGTTGAGCCTGTCGACGCCCGCCCCGCTGCTGACCGAGGACTGACCACTCGACCAGGGTCTGGGTTGACAAACATCAAGACAGAAGAGATCTCAGAAGTGAAGATGGATGCGGAGTTCGGACATGATTCAGGCTTCGAAGTCCGCCATCAAAAACTGGTGTTCTTTGCAGAAGATGTGGGTTCAAACAAAGGTGCCATCATTGGACTCATGGTGGGTGGCGTTGTCATAGCAACAGTGATTGTCATCACCTTGGTGATGCTGAAGAAGAAACAGTACACATCCATCCATCATGGCGTGGTGGAGGTTGACGCTGCTGTGACCCCGGAGGAGCGCCACCTCTCCAAGATGCAGCAGAATGGATATGAGAATCCAACATACAAGTTCTTTGAGCAGATGCAGAACACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Protein Sequence

MLPSLALLLLAAWTVRALEVPTDGNAGLLAEPQIAMFCGKLNMHMNVQNGKWESDPSGTKTCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRGRKQCKTHTHIVIPYRCLVGEFVSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCCPLAEESDSIDSADAEEDDSDVWWGGADTDYADGGEDKVVEVAEEEEVADVEEEEAEDDEDVEDGDEVEEEAEEPYEEATERTTSIATTTTTTTESVEEVVREVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGNRNNFDTEEYCMAVCGSVSSQSLLKTTSEPLPQDPVKLPTTAASTPDAVDKYLETPGDENEHAHFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHFEHVRMVDPKKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISEPRISYGNDALMPSLTETKTTVELLPVNGEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQNTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Signal Transduction; Neurotransmission

Relevant Diseases

Neurodegenerative Disease; Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C

Research Use Only

For research use only

Target

APP

Official Name

APP

Full Name

Amyloid Precursor Protein

Alternative Names

APP; AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; amyloid beta precursor protein; preA4

Gene ID

351 (Human); 11820 (Mouse); 54226 (Rat)

Uniprot ID

P05067 (Human); P12023 (Mouse); P08592 (Rat)