Product
Mouse amyloid beta peptide 1-40, Aβ1-40 ELISA Kit
[CAT#: NRZP-0722-ZP208]
For the quantitative determination of mouse amyloid beta peptide 1-40 (Aβ1-40) concentrations in serum, plasma, tissue homogenates.
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of mouse Aβ1-40. No significant cross-reactivity or interference between mouse Aβ1-40 and analogues was observed.
Species Reactivity
Mouse
Marker
amyloid beta peptide 1-40,Aβ1-40
Principle
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Aβ1-40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Aβ1-40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Aβ1-40 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Aβ1-40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Properites
Sensitivity
7.8 pg/ml
Detection Method
Sandwich
Sample Type
serum, plasma, tissue homogenates
Assay Type
quantitative
Assay Time
3-5 working days
Assay Duration
1-5h
Precision
Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Number Of Samples
50-100ul
Detection Range
31.25 pg/ml-2000 pg/ml
Max Emission
450 nm
Target Details
Publications
Publications (0)
Neural Genes
Neural Antibodies
Kits
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
