ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

Name: ECM Gel from Engelbreth-Holm-Swarm murine sarcoma
SKU: NCC-2104-P03
Rating: 5 (1 reviews)

[CAT#: NCC-2104-P03]

Applications:: Cell Culture

Cell Types:: Neural Lineage Cell

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Product Overview

Description

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.

Cell Types

Neural Lineage Cell

Applications

Cell Culture

Application Notes

ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.

Properties

Components

ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.

Product contains 50 mg/L Gentamicin Sulfate.