Estem™ Neural Progenitor Expansion Kit (Human)
[CAT#: NCC200523ZP]
For the expansion of Human Neural Progenitor Cells
- Applications:
- Cell Expansion; Stem Cell Culture
- Cell Types:
- Neural Stem Cell; Neural Progenitor Cell
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The human neural progenitor cell expansion kit contains human neural progenitor cells and an optimized expansion medium. Human neural progenitor cells are derived from NIH approved H9 human embryonic stem cells (hESCs). These neural progenitor cells derived from hESC proliferate in the form of a monolayer of adherent cells and can easily differentiate into different neuron subtypes. Spectral analysis showed that after ten passages, ENStem-A cells retained the normal diploid karyotype in culture. It can be used for various research applications, such as neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor function. ENStem-A nerve expansion medium is a clear serum-free preparation. When used in combination with FGF-2 (included in the kit), the expansion medium will allow the maintenance and proliferation of human neural progenitor cells.
Cell Types
Neural Stem Cell; Neural Progenitor Cell
Species Reactivity
Human
Applications
Cell Expansion; Stem Cell Culture
Application Notes
The human neural progenitor cell expansion kit contains human neural progenitor cells and an optimized expansion medium.
Relevant Diseases
Neurological Disorders
Research Areas
Stem Cell Research
Properties
Components
Human Neural Progenitors, 1 x 10e6 cells
Neural Expansion Media, SCM004, 500 mls
Basic FGF, lyophilized, 10 ug
Neural Expansion Media, SCM004, 500 mls
Basic FGF, lyophilized, 10 ug
Form
Liquid
Serum Level
Serum-Free
Shipping
Dry ice
Storage
Cells: When stored under the recommended storage conditions (liquid nitrogen), cells are stable up to five years. Do not expose to high temperature. After expiration, discard all remaining reagents. We recommend using cells within ten generations.
Medium: Medium should be stored at -20 °C, until ready to use. After thawing, the medium should be stored at 2-8C, and given a 1-month expiration dating. It is recommended to divide into small portions to avoid repeated heating before each use.
Medium: Medium should be stored at -20 °C, until ready to use. After thawing, the medium should be stored at 2-8C, and given a 1-month expiration dating. It is recommended to divide into small portions to avoid repeated heating before each use.
Handling Advices
Store under recommended storage conditions. Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
It has been verified that each batch of human neural progenitor cells express Nestin and Sox 2 at high levels, and Oct-4 at low levels. The ability of ENStem-A cells to differentiate into multiple neuron phenotypes and maintain normal karyotype after multiple passages was verified. Mycoplasma is negative.
Shelf Life
Stable until expiry date (EXP) on label.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Neural Cell Culture
For Research Use Only. Not For Clinical Use.
