Neural Progenitor Expansion Kit (Human)

[CAT#: NCC200523ZP]

For the expansion of Human Neural Progenitor Cells

Applications:: Cell Expansion; Stem Cell Culture

Cell Types:: Neural Stem Cell; Neural Progenitor Cell

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Product Overview

Description

The expansion kit for human neural progenitor cells includes these cells and a specially designed medium for their growth. These progenitor cells are sourced from NIH-endorsed H9 human embryonic stem cells (hESCs). They grow as a layer of adherent cells, capable of smoothly transitioning into various neuron types. Further examination revealed that even after ten passages, these neural progenitor cells maintained a normal diploid karyotype in culture. This kit is versatile for a range of neuroscientific studies, including neurotoxicity, neurogenesis, electrophysiology, neurotransmitter, and receptor assessments. The serum-free medium provided supports the sustained growth and expansion of these human neural progenitor cells alongside FGF-2 supplementation, which is incorporated in the kit.

Species Reactivity

Human

Cell Types

Neural Stem Cell; Neural Progenitor Cell

Applications

Cell Expansion; Stem Cell Culture

Application Notes

The human neural progenitor cell expansion kit contains human neural progenitor cells and an optimized expansion medium.

Relevant Diseases

Neurological Disorders

Research Areas

Stem Cell Research

Properites

Components

Human Neural Progenitors, 1 x 10e6 cells
Neural Expansion Media, 500 mls
Basic FGF, 10 ug

Form

Liquid

Serum Level

Serum-Free

Preparation and Storage

Shipping

Dry ice

Storage

Cells: When stored under the recommended storage conditions (liquid nitrogen), cells are stable up to five years. Do not expose to high temperature. After expiration, discard all remaining reagents. We recommend using cells within ten generations.

Medium: Medium should be stored at -20 °C, until ready to use. After thawing, the medium should be stored at 2-8 °C, and given a 1-month expiration dating. It is recommended to divide into small portions to avoid repeated heating before each use.

Handling Advices

Store under recommended storage conditions. Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Each batch of human neural progenitor cells express Nestin and Sox2 at high levels, and Oct-4 at low levels. The ability of cells to differentiate into multiple neuron phenotypes and maintain normal karyotype after multiple passages was verified. No mycoplasma contamination.