Human APH1A Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP190]

Human APH1A knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human APH1A has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and 7 bp deletion in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neural Signal Transduction

Pathways

Notch Pathway

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

APH1A

Official Name

APH1A

Full Name

Aph-1 Homolog A, Gamma-Secretase Subunit

Alternative Names

Aph-1 Homolog A; Gamma-Secretase Subunit; Presenilin-Stabilization Factor; APH1A Gamma Secretase Subunit; Aph-1alpha; APH-1A; Anterior Pharynx Defective 1 Homolog A (C. Elegans); Anterior Pharynx Defective 1 Homolog A;