Product

Human ASIC1 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP104]

Human ASIC1 knockout cell lysate

Species:: Human

Cell Types:: HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human ASIC1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Pathways

Sodium Signaling

Marker

Sodium Channels

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

ASIC1

Official Name

ASIC1

Full Name

Acid Sensing Ion Channel Subunit 1

Alternative Names

Acid Sensing Ion Channel Subunit 1; Amiloride-Sensitive Cation Channel 2; Neuronal; Acid Sensing (Proton Gated) Ion Channel 1; Brain Sodium Channel 2; ACCN2; BNaC2;